Anti-Human F3 Recombinant Antibody (TAB-473CQ)
CAT#: TAB-473CQ
Recombinant monoclonal antibody to F3. This product is a human monoclonal antibody that targets human F3.
Tested Data
Gene Expression
WB
Figure 1 Anti-Human F3 Antibody (TAB-473CQ) in WB
Western blot analysis of TAB-473CQ was performed by loading human F3 protein in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-473CQ (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Chemiluminescent detection was performed.
DB
Figure 2 Anti-Human F3 Antibody (TAB-473CQ) in DB
Dot Blot analysis of TAB-473CQ was performed by coating with human F3 protein.
TAB-473CQ incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
ELISA
Figure 3 Anti-Human F3 Antibody (TAB-473CQ) in ELISA
ELISA analysis of TAB-473CQ was performed by coating with human F3 protein. Then blocked with BSA and incubated with TAB-473CQ. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49292/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69132/1343_C8_1_selected.jpg
Subcellular Location
Figure 3 IHC staining of human nasopharynx
Immunohistochemical staining of human nasopharynx shows strong cytoplasmic positivity in respiratory epithelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_B_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_A_6_6.jpg
Normal Tissue
Figure 6 Placenta
Decidual cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_A_2_7.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A-431, HaCaT, HBEC3-KT, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117525-F3
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG1, κ
- Specificity
- Human F3
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry; Inhibition
- Conjugate
- Unconjugated
- Related Disease
- Solid tumors
Product Property
- Purity
- > 95% as determined by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
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Broad Application Spectrum
From IP to flow cytometry, the antibody's broad applicability supports diverse research needs. This versatility enhances our ability to adapt to various experimental designs, significantly broadening the scope of our scientific investigations.
Excellent Sensitivity
The sensitivity of the Anti-Human F3 antibody is exceptional, particularly in ELISA and immunohistochemistry applications. It consistently provides us with high-quality, reproducible data essential for our coagulation studies, ensuring confidence in the results.
Q&As
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What research applications can the Anti-Human F3 Recombinant Antibody support?
A: This antibody is designed for use in various immunoassays including ELISA, immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP), and inhibition studies. These applications facilitate exploration into pathophysiological roles relating to coagulation and tumor biology.
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Which diseases or conditions are associated with the F3 target?
A: F3, also known as tissue factor, is significant in coagulation and is often implicated in solid tumors. This antibody aids the detailed investigation of F3's role in the coagulation cascade, hemostatic processes, and cancer progression.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-473CQ, RRID: AB_3111961)
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Biosimilar Overview
Please refer to Tisotumab Vedotin Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Tisotumab Vedotin.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0573CQ | Mouse Anti-F3 Recombinant Antibody (clone hATR-5) | ELISA, Inhib | Mouse IgG4 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-473CQ | Afuco™ Anti-F3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-473CQ) | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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